NDLI: Synthesis of pyrophosphate by chromatophores of Rhodospirillum rubrum in the light and by soluble yeast inorganic pyrophosphatase in water-organic solvent mixtures

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Synthesis of pyrophosphate by chromatophores of Rhodospirillum rubrum in the light and by soluble yeast inorganic pyrophosphatase in water-organic solvent mixtures

Content Provider	Scilit
Author	Behrens, Maria Isabel Meis, Leopoldo
Copyright Year	1985
Description	Journal: Journal of Biological Inorganic Chemistry Chromatophores of Rhodospirillum rubrum contain a membrane-bound pyrophosphatase that synthesizes pyrophosphate when an electrochemical H+ gradient is formed across the chromatophore membrane upon illumination. In this report it is shown that MgCl2 and Pi have different effects on the synthesis of pyrophosphate in the light depending on whether initial velocities or steady-state levels are examined. When the water activity of the medium is reduced by the addition of organic solvents, soluble yeast inorganic pyrophosphatase (no H+ gradient present) synthesizes pyrophosphate in amounts similar to those synthesized by the chromatophores in totally aqueous medium during illumination, (H+ gradient present). The pH, MgCl2 and Pi dependence for the synthesis of pyrophosphate by the chromatophores at steady-state is similar to that observed at equilibrium with the soluble enzyme in the presence of organic solvents. The possibility is raised that a decrease in water activity may play a role in the mechanism by which the energy derived from the electrochemical H+ gradient is used for the synthesis of pyrophosphate in chromatophores of R. rubrum.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1985.tb09187.x/pdf
Ending Page	227
Page Count	7
Starting Page	221
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1985.tb09187.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	1
Volume Number	152
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1985-10-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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