Loading...
Please wait, while we are loading the content...
Similar Documents
Homologous RNA Polymerase binding to Escherichia coli DNA. A Study of the Distribution of Stable Binding Sites
| Content Provider | Scilit |
|---|---|
| Author | Pedone, Francesco Ballario, Paola Mauro, Ernesto |
| Copyright Year | 1978 |
| Description | Journal: Journal of Biological Inorganic Chemistry The number and the distribution of the sites of Escherichia coli DNA that form stable complexes with the homologous RNA polymerase (class A sites according to Hinkle and Chamberlin [3]) have been investigated. Almost all the DNA can bind RNA polymerase, even when fragmented at short (undergenic) size; this general non-promoter-specific binding is highly labile and is not temperature-dependent. The range of RNA polymerase/DNA ratios that give rise to the stable temperature-dependent complexes was examined. The amount and the distribution of class A complexes were studied analysing the dissociation of complexes formed by RNA polymerase on DNA fragments of various length. The E. coli genome appears to form 3.8 X 10(3) stable complexes; the majority of these complexes shows a short-range distribution (800-1200 base pairs). The rest is more widely spaced (1200-6000 base pairs). |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12394.x/pdf |
| Ending Page | 451 |
| Page Count | 7 |
| Starting Page | 445 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1978.tb12394.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 3 |
| Volume Number | 87 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1978-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Escherichia Coli Binding Sites Homologous Rna Rna Polymerase Binding Stable Binding |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
