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Pharmacological Characterization of Cholinergic Receptors in a Human Neuroblastoma Cell Line
| Content Provider | Scilit |
|---|---|
| Author | Clementi, F. Cabrini, D. Gotti, C. Sher, E. |
| Copyright Year | 1986 |
| Description | Journal: Journal of Neurochemistry A human neuroblastoma cell line, IMR32, has been characterized as far as morphology, membrane receptors for neurotransmitters, and uptake and release of $[^{3}$H]3,4-dihydroxyphenylethylamine $([^{3}$H]dopamine). These cells expressed at their surface both nicotinic and muscarinic cholinergic receptors, revealed by $[^{125}$I]α-bungarotoxin and $[^{3}$H]quinuclidinylbenzilate $([^{3}$H]QNB) binding, respectively. $[^{125}$I]α-Bungarotoxin binding was efficiently inhibited by α-bungarotoxin, nicotine, carbachol, and d-tubocurarine. $[^{3}$H]QNB binding was competitively inhibited by atropine, pirenzepine, and carbachol. Hexamethonium did not affect the binding of either ligand. In competition experiments with $[^{3}$H]QNB, pirenzepine recognized only one binding site with “low affinity,” and carbachol recognized two sites with different affinities, β-adrenergic receptors were present in a very low amount, whereas α-adrenergic and dopaminergic receptors were not detectable. IMR32 cells had an imipramine-sensitive $[^{3}$H]dopamine uptake, but carbachol, high levels of $K^{+}$, the calcium ionophore A23187, and α-latrotoxin were not able to induce release of $[^{3}$H]dopamine that had been taken up. The ultrastructural analysis showed that IMR32 cells contained very few dense-core vesicles, suggesting a low storage capacity for neurotransmitter. These cells could be an useful in vitro model for studying neurotransmitter receptors of the human CNS. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.1986.tb02861.x |
| Ending Page | 297 |
| Page Count | 7 |
| Starting Page | 291 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1986.tb02861.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 1 |
| Volume Number | 47 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1986-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology Imr32 Neuroblastoma Quinuclidinylbenzilate |
| Content Type | Text |
| Resource Type | Article |
