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Crystal structures of the human SUMO-2 protein at 1.6 Å and 1.2 Å resolution
| Content Provider | Scilit |
|---|---|
| Author | Huang, Wen-Chen Ko, Tzu-Ping Li, Steven S. -L Wang, Andrew H. -J. |
| Copyright Year | 2004 |
| Description | Journal: Journal of Biological Inorganic Chemistry The SUMO proteins are a class of small ubiquitin-like modifiers. SUMO is attached to a specific lysine side chain on the target protein via an isopeptide bond with its C-terminal glycine. There are at least four SUMO proteins in humans, which are involved in protein trafficking and targeting. A truncated human SUMO-2 protein that contains residues 9-93 was expressed in Escherichia coli and crystallized in two different unit cells, with dimensions of a=b=75.25 A, c=29.17 A and a=b=74.96 A, c=33.23 A, both belonging to the rhombohedral space group R3. They diffracted X-rays to 1.6 A and 1.2 A resolution, respectively. The structures were determined by molecular replacement using the yeast SMT3 protein as a search model. Subsequent refinements yielded R/Rfree values of 0.169/0.190 and 0.119/0.185, at 1.6 A and 1.2 A, respectively. The peptide folding of SUMO-2 consists of a half-open beta-barrel and two flanking alpha-helices with secondary structural elements arranged as betabetaalphabetabetaalphabeta in the sequence, identical to those of ubiquitin, SMT3 and SUMO-1. Comparison of SUMO-2 with SUMO-1 showed a surface region near the C terminus with significantly different charge distributions. This may explain their distinct intracellular locations. In addition, crystal-packing analysis suggests a possible trimeric assembly of the SUMO-2 protein, of which the biological significance remains to be determined. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.2004.04349.x/pdf |
| Ending Page | 4122 |
| Page Count | 9 |
| Starting Page | 4114 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.2004.04349.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 20 |
| Volume Number | 271 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2004-09-24 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Homology Modeling Molecular Interactions Protein Modification Surface Charge Distributions Synchrotron Radiations |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
