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Poly-beta-hydroxyalkanoates consumption during degradation of 2,4,6-trichlorophenol by Sphingopyxis chilensis S37
| Content Provider | Scilit |
|---|---|
| Author | Godoy, Felix Bunster, M. Matus, V. Aranda, Carlos Gonzalez, B. Martinez, M. A. |
| Copyright Year | 2003 |
| Description | Journal: Letters in applied microbiology To analyse the possible effect of poly-beta-hydroxyalkanoate (PHA) consumption on 2,4,6-trichlorophenol (2,4,6-TCP) degradation during starvation by Sphingopyxis chilensis S37 strain, which stores PHAs and degrades 2,4,6-TCP. The strain was inoculated in saline solution supplemented with 2,4,6-TCP (25-400 microm). Chlorophenol degradation was followed both spectrophotometrically and by chlorine released; viable bacterial counts were also determined. Cells starved for 24, 48 or 72 h were incubated with 25 microm of 2,4,6-TCP and PHA in cells investigated by spectrofluorimetric and flow cytometry. Results demonstrated that starvation decreased the ability to degrade 2,4,6-TCP. After 72 h of starvation, degradation of 2,4,6-TCP decreased to less than 10% and the relative PHA content diminished to ca 50% during the first 24 h. Utilization of PHA may be an important factor for the degradation of toxic compounds, such as 2,4,6-TCP, in bacterial strains unable to use this toxic compound as carbon and energy source. This is the first study describing a relationship between intracellular PHA consumption and 2,4,6-TCP degradation. Therefore, PHAs provides an endogenous carbon and energy source under starvation and can play a significant role in the degradation of toxic compounds. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1046/j.1472-765X.2003.01315.x/pdf |
| Ending Page | 320 |
| Page Count | 6 |
| Starting Page | 315 |
| e-ISSN | 1472765X |
| DOI | 10.1046/j.1472-765x.2003.01315.x |
| Journal | Letters in applied microbiology |
| Issue Number | 5 |
| Volume Number | 36 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2003-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Biotechnology and Microbiology Hydroxyalkanoates |
| Content Type | Text |
| Resource Type | Article |
