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High level synthesis of biologically active recombinant trichosanthin in Escherichia coli
| Content Provider | Scilit |
|---|---|
| Author | Zhu, Rong-Huan Ng, Tzi-Bun Yeung, Hin-Wing Shaw, Pang-Chui |
| Copyright Year | 1992 |
| Description | Journal: International journal of peptide and protein research Two forms of recombinant trichosanthin (rTCS) were synthesized in high levels in Escherichia coli by putting the TCS cDNA under the control of a T7 RNA polymerase-directed promoter. Purification schemes were developed to isolate the recombinant protein from both soluble and insoluble fractions. Form I rTCS possessed the mature TCS sequence and had similar biological activities as the natural protein. Its $IC_{50}$ was approximately 0.13 nm in an in vitro rabbit reticulocyte translational system and a dose of around 35 μg protein per 25 g body weight was sufficient to induce complete abortion in mice. Form II rTCS had a propeptide of 19 aa at the C-terminus and was five times less active than Form I in inhibiting protein synthesis by a rabbit reticulocyte lysate. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3011.1992.tb01558.x |
| Ending Page | 81 |
| Page Count | 5 |
| Starting Page | 77 |
| ISSN | 03678377 |
| DOI | 10.1111/j.1399-3011.1992.tb01558.x |
| Journal | International journal of peptide and protein research |
| Issue Number | 1 |
| Volume Number | 39 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1992-01-12 |
| Access Restriction | Open |
| Subject Keyword | Journal: International journal of peptide and protein research Biotechnology and Microbiology Recombinant Protein Ribosome-inactivating Protein |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
