NDLI: Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood

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Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood

Content Provider	Scilit
Author	Moriyama, Kosei Ando, Chie Tashiro, Kosuke Kuhara, Satoru Okamura, Seiichi Nakano, Shuji Takagi, Yasumitsu Miki, Takeyoshi Nakashima, Yoshiyuki Hirakawa, Hideki
Copyright Year	2008
Description	Journal: Microbiology and Immunology Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aqꮬterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.2008.00048.x/pdf
Ending Page	382
Page Count	8
Starting Page	375
ISSN	03855600
e-ISSN	13480421
DOI	10.1111/j.1348-0421.2008.00048.x
Journal	Microbiology and Immunology
Issue Number	7
Volume Number	52
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2008-07-01
Access Restriction	Open
Subject Keyword	Journal: Microbiology and Immunology Analytical Chemistry Polymerase Chain Reaction 16s Ribosomal Rna
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology

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