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Development of high transferability cpSSR markers for individual identification and genetic investigation in Cupressaceae species
| Content Provider | Scilit |
|---|---|
| Author | Huang, Li-Sha Sun, Yan-Qiang Jin, Yuqing Gao, Qiong Hu, Xian-Ge Gao, Fu-Ling Yang, Xiao-Lei Zhu, Ji-Jun El-Kassaby, Yousry A. Mao, Jian‐Feng |
| Copyright Year | 2018 |
| Description | Journal: Ecology and Evolution Given the low substitution rate in plastomes, the polymorphic and codominant nature of chloroplast SSRs (cpSSRs) makes them ideal markers, complementing their nuclear counterpart. In Cupressaceae, cpSSRs are mostly paternally inherited, thus, they are useful in mating systems and pollen flow studies. Using e‐PCR, 92 SSR loci were identified across six Cupressaceae plastomes, and primers were designed for 26 loci with potential interspecific transferability. The 26 developed cpSSRs were polymorphic in four genera, Platycladus, Sabina, Juniperus, and Cupressus and are suitable for Cupressaceae molecular genetic studies and utilization. We genotyped 192 Platycladus orientalis samples from a core breeding population using 10 of the developed cpSSRs and 10 nuclear SSRs, and these individuals were identified with high confidence. The developed cpSSRs can be used in (1) a marker‐assisted breeding scheme, specifically when paternity identification is required, (2) population genetics investigations, and (3) biogeography of Cupressaceae and unraveling the genetic relationships between related species. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5980425/pdf |
| Ending Page | 4977 |
| Page Count | 11 |
| Starting Page | 4967 |
| ISSN | 20457758 |
| e-ISSN | 20457758 |
| DOI | 10.1002/ece3.4053 |
| Journal | Ecology and Evolution |
| Issue Number | 10 |
| Volume Number | 8 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2018-04-20 |
| Access Restriction | Open |
| Subject Keyword | Journal: Ecology and Evolution Genetic Diversity |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ecology Ecology, Evolution, Behavior and Systematics Nature and Landscape Conservation |
