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Unfolding and refolding of cytochromecdriven by the interaction with lipid micelles
| Content Provider | Scilit |
|---|---|
| Author | Sanghera, Narinder Pinheiro, Teresa J. T. |
| Copyright Year | 2000 |
| Description | Journal: Protein Science Binding of native cyt c to L-PG micelles leads to a partially unfolded conformation of cyt c. This micelle-bound state has no stable tertiary structure, but remains as alpha-helical as native cyt c in solution. In contrast, binding of the acid-unfolded cyt c to L-PG micelles induces folding of the polypeptide, resulting in a similar helical state to that originated from the binding of native cyt c to L-PG micelles. Far-ultraviolet (UV) circular dichroism (CD) spectra showed that this common micelle-associated helical state (HL) has a native-like alpha-helix content, but is highly expanded without a tightly packed hydrophobic core, as revealed by tryptophan fluorescence, near-UV, and Soret CD spectroscopy. The kinetics of the interaction of native and acid-unfolded cyt c was investigated by stopped-flow tryptophan fluorescence. Formation of H(L) from the native state requires the disruption of the tightly packed hydrophobic core in the native protein. This micelle-induced unfolding of cyt c occurs at a rate approximately 0.1 s(-1), which is remarkably faster in the lipid environment compared with the expected rate of unfolding in solution. Refolding of acid-unfolded cyt c with L-PG micelles involves an early highly helical collapsed state formed during the burst phase (<3 ms), and the observed main kinetic event reports on the opening of this early compact intermediate prior to insertion into the lipid micelle. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2144667/pdf |
| Ending Page | 1202 |
| Page Count | 9 |
| Starting Page | 1194 |
| e-ISSN | 1469896X |
| DOI | 10.1110/ps.9.6.1194 |
| Journal | Protein Science |
| Issue Number | 6 |
| Volume Number | 9 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2000-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Protein Science Biochemistry and Molecular Biology Folding in Lipid Membranes Folding Intermediates Membrane Insertion Collapsed State Folding Kinetics Cytochrome C Unfolding in Lipid Membranes |
| Content Type | Text |
| Resource Type | Article |
