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Release of Catecholamines from Superfused Bovine Adrenal Chromaffin Cells Cultured on Microcarrier Beads
| Content Provider | Scilit |
|---|---|
| Author | Boarder, M. R. McArdle, W. |
| Copyright Year | 1986 |
| Description | Journal: Journal of Neurochemistry A procedure is described for the establishment of stable primary cultures of bovine chromaffin cells on microcarrier beads. The cells flatten and send out processes with varicosities over a few days and maintain their catecholamine content for 2 weeks. The beads may be incorporated into a superfusion apparatus with a chamber volume of about 150 microliters, enabling the efficient perfusion of a high density of cells. The response to the introduction of nicotine and high potassium into the perfusing medium is shown to be more rapid and more transient than hitherto described, with each secretagogue producing a different degree of preferential stimulation of noradrenaline-secreting cells. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1986.tb01764.x/pdf |
| Ending Page | 1477 |
| Page Count | 5 |
| Starting Page | 1473 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1986.tb01764.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 5 |
| Volume Number | 46 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1986-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemical Research |
| Content Type | Text |
| Resource Type | Article |
