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Nucleotide Sequence (n = 159) of the Amino-Acid-Accepting 3'-OH Extremity of Turnip-Yellow-Mosaic-Virus RNA and the Last Portion of Its Coat-Protein Cistron
| Content Provider | Scilit |
|---|---|
| Author | Briand, Jean-Paul Jonard, Gerard Guilley, Hubert Richards, Kenneth Hirth, Leon |
| Copyright Year | 1977 |
| Description | Journal: Journal of Biological Inorganic Chemistry The experiments described in this paper and the following one establish the sequence of the 3'-OH terminal 159 nucleotides of turnip yellow mosaic virus RNA. Uniformly 32P-labeled turnip yellow mosaic virus RNA was partially digested with T1 ribonuclease and the fragments were fractionated by polyacrylamide gel electrophoresis. Fragments originating from the 3'-OH end of the RNA molecule were identified by testing for the 3'-terminal oligonucleotide, C-COH, after total U2 ribonuclease hydrolysis. Once identified, the 3'-OH terminal fragments were sequenced by the methods of Sanger et al. The first 51 nucleotides of the longest of the sequenced fragments (158 nucleotides) extends into the 3'-terminal part of the coat protein cistron. The coat protein cistron is followed by a stretch of 108 untranslated nucleotides whose function, though still unknown, is probably linked to the tRNA-like properties which have been attributed to the 3'-OH extremity of this viral RNA. Two possible secondary structures are proposed for the sequence and the implications of the findings with regard to the tRNA-like properties of the extremity are discussed. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1977.tb11269.x/pdf |
| Ending Page | 463 |
| Page Count | 11 |
| Starting Page | 453 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1977.tb11269.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 3 |
| Volume Number | 72 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1977-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Amino Acid Nucleotide Sequence |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
