Loading...
Please wait, while we are loading the content...
Similar Documents
A Characterization of Alfalfa-Mosaic-Virus Protein Polymerization in the Presence of Nucleic Acid
| Content Provider | Scilit |
|---|---|
| Author | Driedonks, René A. Krijgsman, Peter C. J. Mellema, Jan E. |
| Copyright Year | 1978 |
| Description | Journal: Journal of Biological Inorganic Chemistry The polymerization of alfalfa mosaic virus (AMV) protein in the presence of homologous nucleic acids and a number of other natural and synthetic nucleic acids was studied. The conditions for optimal assembly were found to be pH 6.0 and low ionic strength (I = 0.1 M) at room temperature, irrespective of the type of nucleic acid. The resulting nucleoprotein particles exhibited the same structural characteristics as the virus. This information emerged from optical diffraction and computer filtering of electron micrographs from the reconstituted particles. Irrespective of the type of nucleic acid present the polymerization of the protein resulting in a nucleoprotein particle is a cooperative process. Evidence for this was obtained by nitrocellulose filter binding assay, sodium dodecylsulphate/polyacrylamide gel electrophoresis, sedimentation velocity and electron microscopy of the reaction mixtures. The rates and efficiencies of reconstitution were of the same order of magnitude for a number of ribonucleic acids. Sedimentation data derived from AMV protein and AMV RNA mixtures suggested the existence of a specific nucleation product in the first stage of assembly. The results are discussed in terms of a tentative model of the assembly, in which at least two different steps (nucleation and elongation) can be distinguished, each characterized by an association constant. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1978.tb12035.x/pdf |
| Ending Page | 417 |
| Page Count | 13 |
| Starting Page | 405 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1978.tb12035.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 82 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1978-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Nucleic Acid Protein Polymerization Mosaic Virus Alfalfa Mosaic Characterization of Alfalfa |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
