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Dominant Prostasome Immunogens for Sperm-Agglutinating Autoantibodies of Infertile Men
| Content Provider | Scilit |
|---|---|
| Author | Carlsson, Lena Ronquist, Gunnar Nilsson, B. Ove Larsson, Anders |
| Copyright Year | 2004 |
| Description | Journal: Journal of Andrology The presence of naturally occurring anti‐sperm antibodies (ASA) is a well‐known cause of infertility in men and women, but the antigens for these antibodies are poorly characterized. We have previously shown that prostasomes adhere to sperm cells and that prostasomes are major targets for ASA associated with infertility. These autoantigens have not been characterized. We used 2‐dimensional electrophoresis, immunoblotting, and mass‐spectrometry to identify the prostasome antigens for these autoantibodies. By these techniques, we revealed that prolactin‐inducible protein (PIP) and clusterin were dominant prostasome immunogens for spermagglutinating autoantibodies of 20 patients with immunological infertility. PIP was identified by 19 of 20 (95%) patient sera and clusterin by 17 of 20 (85%). In addition, 10 sporadically occurring prostasomal antigens were identified in this context, viz alcohol dehydrogenase $[NADP^{+}$], annexin I, annexin III, BRCA1‐associated ring domain protein 1, heat shock 27‐kd protein, isocitrate dehydrogenase, lactoylglutathione lyase, NG,NG‐dimethylarginine dimethylaminohydrolase 1, peroxiredoxin 2, and syntenin 1. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.1939-4640.2004.tb02844.x |
| Ending Page | 705 |
| Page Count | 7 |
| Starting Page | 699 |
| ISSN | 01963635 |
| e-ISSN | 19394640 |
| DOI | 10.1002/j.1939-4640.2004.tb02844.x |
| Journal | Journal of Andrology |
| Issue Number | 5 |
| Volume Number | 25 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2004-09-10 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Andrology Reproductive Biology Antisperm Antibodies (asa) |
| Content Type | Text |
| Subject | Endocrinology, Diabetes and Metabolism Urology Endocrinology Reproductive Medicine |
