Loading...
Please wait, while we are loading the content...
Similar Documents
Nitric oxide-releasing flurbiprofen reduces formation of proinflammatory hydrogen sulfide in lipopolysaccharide-treated rat
| Content Provider | Scilit |
|---|---|
| Author | Anuar, Farhana Whiteman, Matthew Siau, Jia Ling Kwong, Shing Erl Bhatia, Madhav Moore, Philip K. |
| Copyright Year | 2006 |
| Description | Journal: British Journal of Pharmacology The biosynthesis of both nitric oxide (NO) and hydrogen sulfide (H2S) is increased in lipopolysaccharide (LPS)-injected mice and rats but their interaction in these models is not known. In this study we examined the effect of the NO donor, nitroflurbiprofen (and the parent molecule flurbiprofen) on NO and H2S metabolism in tissues from LPS-pretreated rats. Administration of LPS (10 mg kg(-1), i.p.; 6 h) resulted in an increase (P<0.05) in plasma TNF-alpha, IL-1beta and nitrate/nitrite (NO(x)) concentrations, liver H2S synthesis (from added cysteine), CSE mRNA, inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO) activity (marker for neutrophil infiltration) and nuclear factor-kappa B (NF-kappaB) activation. Nitroflurbiprofen (3-30 mg kg(-1), i.p.) administration resulted in a dose-dependent inhibition of the LPS-mediated increase in plasma TNF-alpha, IL-1beta and NO(x) concentration, liver H2S synthesis (55.00+/-0.95 nmole mg protein(-1), c.f. 62.38+/-0.47 nmole mg protein(-1), n = 5, P<0.05), CSE mRNA, iNOS, MPO activity and NF-kappaB activation. Flurbiprofen (21 mg kg(-1), i.p.) was without effect. These results show for the first time that nitroflurbiprofen downregulates the biosynthesis of proinflammatory H2S and suggest that such an effect may contribute to the augmented anti-inflammatory activity of this compound. These data also highlight the existence of 'crosstalk' between NO and H2S in this model of endotoxic shock. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1760720/pdf |
| Ending Page | 974 |
| Page Count | 9 |
| Starting Page | 966 |
| e-ISSN | 14765381 |
| DOI | 10.1038/sj.bjp.0706696 |
| Journal | British Journal of Pharmacology |
| Issue Number | 8 |
| Volume Number | 147 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2006-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: British Journal of Pharmacology Nitroflurbiprofen Hydrogen Sulphide Lipopolysaccharide-induced Endotoxic Shock Tumour Necrosis Factor-α Cystathionine Γ Lyase |
| Content Type | Text |
| Resource Type | Article |
