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A complex fruit-specific type-2 ribosome-inactivating protein from elderberry (Sambucus nigra) is correctly processed and assembled in transgenic tobacco plants
| Content Provider | Scilit |
|---|---|
| Author | Chen, Ying Vandenbussche, Frank Rougé, Pierre Proost, Paul Peumans, Willy J. Damme, Els J. M. Van |
| Copyright Year | 2002 |
| Description | Journal: Journal of Biological Inorganic Chemistry Fruits of elderberry (Sambucus nigra) express small quantities of a type‐2 ribosome‐inactivating protein with an exclusive specificity towards the NeuAc(α2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B‐chains of two adjacent protomers. A cDNA clone encoding this so‐called Sambucus nigra fruit specific agglutinin I (SNA‐If) was isolated and expressed in tobacco (Samsun NN) under the control of the 35S cauliflower mosaic virus promoter. Characterization of the purified protein indicated that the recombinant SNA‐If from tobacco leaves has the same molecular structure and biological activities as native SNA‐If from elderberry fruits, demonstrating that transgenic tobacco plants are fully capable of expressing and correctly processing and assembling a type‐2 ribosome‐inactivating protein with a complex molecular structure. None of the transformants showed a phenotypic effect, indicating that the ectopically expressed SNA‐If does not affect the viability of the tobacco cells. Bioassays further demonstrated that none of the transgenic lines exhibited a decreased sensitivity to infection with tobacco mosaic virus suggesting that the elderberry type‐2 RIP SNA‐If does not act as an antiviral agent in planta. |
| Ending Page | 2906 |
| Starting Page | 2897 |
| ISSN | 2573508X |
| e-ISSN | 14321327 |
| DOI | 10.1046/j.1432-1033.2002.02962.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 12 |
| Volume Number | 269 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2002-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Plant Sciences Inactivating Protein |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
