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Gene targeting demonstrates that inducible nitric oxide synthase is not essential for resistance to oral candidiasis in mice, or for killing ofCandida albicansby macrophagesin vitro
| Content Provider | Scilit |
|---|---|
| Author | Farah, C. S. Saunus, Jodi Hu, Y. Kazoullis, Andrea Ashman, R. B. |
| Copyright Year | 2009 |
| Description | Journal: Oral microbiology and immunology Oral candidiasis is caused by opportunistic infections with the yeast Candida albicans. Previous studies have demonstrated important roles for innate immunity and T helper type 1-mediated inflammatory reactions in recovery from infection, with macrophages and neutrophils as key effector cells. Both effector cell types use the inducible isoform of nitric oxide synthase (iNOS) to generate candidacidal molecules, but it is not clear whether nitric oxide (NO) is an absolute requirement for candidacidal effector activity. In this study we directly investigated the role of iNOS-derived NO in resistance to murine experimental oral candidiasis, using iNOS knockout mice. Knockout mice were no more susceptible to oral candidiasis than wild-type controls. Bone marrow-derived macrophages from the knockout mice killed C. albicans yeasts efficiently in vitro, and were still able to produce nitrites in an iNOS-independent manner, albeit less efficiently than wild-type controls. There were no significant differences in local mucosal production of interleukins 6, 12, 17A, or 23, interferon-gamma, or transforming growth factor-beta 24 h after oral challenge with C. albicans. These data suggest that iNOS-derived NO is not required for resistance to oral candidiasis in vivo, and that bone marrow-derived macrophages may have iNOS-independent means of generating reactive nitrogen species. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1399-302X.2008.00462.x/pdf |
| Ending Page | 88 |
| Page Count | 6 |
| Starting Page | 83 |
| ISSN | 09020055 |
| e-ISSN | 1399302X |
| DOI | 10.1111/j.1399-302x.2008.00462.x |
| Journal | Oral microbiology and immunology |
| Issue Number | 1 |
| Volume Number | 24 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2009-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oral microbiology and immunology Candida Albicans Innate Immunity Oral Candidiasis Oral Infection |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) Dentistry Immunology Microbiology |
