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Sodium/Proton Exchange in Cultured Bovine Adrenal Medullary Cells
| Content Provider | Scilit |
|---|---|
| Author | Yanagihara, Nobuyuki Yokota, Kenji Kobayashi, Hideyuki Wada, Akihiko Uezono, Yasuhito Izumi, Futoshi |
| Copyright Year | 1990 |
| Description | Journal: Journal of Neurochemistry We investigated the presence of Na+/H+ exchange in cultured bovine adrenal medullary cells. The intracellular pH in control cells measured by 5,5-dimethyl[2-14C]oxazolidine-2,4-dione was 7.13 +/- 0.02 (n = 6). Removal of Na+ from the incubation medium shifted the intracellular pH down to 6.67 +/- 0.12 (n = 6). Reintroduction of Na+ to the medium caused a rapid recovery in intracellular pH to 7.20-7.30 that was associated with an increase in uptake of 22Na+ by the cells. Both increases in intracellular pH and uptake of 22Na+ were inhibited by amiloride, an inhibitor of Na+/H+ exchange. The recovery of intracellular pH by addition of Na+ was partially inhibited by quinidine, another inhibitor of Na+/H+ exchange, but not by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, an anion-exchange (Cl-/HCO3-) inhibitor. Li+ could substitute for Na+ in the recovery of intracellular pH. Carbachol caused an increase in intracellular pH from 7.12 +/- 0.01 to 7.21 +/- 0.02 (n = 10). This increase in intracellular pH caused by carbachol was inhibited by amiloride. These results suggest the existence of an amiloride-sensitive Na+/H+ exchange that regulates the intracellular pH in adrenal medullary cells. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1990.tb01214.x/pdf |
| Ending Page | 1631 |
| Page Count | 6 |
| Starting Page | 1626 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1990.tb01214.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 5 |
| Volume Number | 54 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1990-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Endocrinology and Metabolism Adrenal Medulla Intracellular Ph 22na+ Uptake Sodium/proton Exchange |
| Content Type | Text |
| Resource Type | Article |
