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Resorcinarene-Based Facial Glycosides: Implication of Detergent Flexibility on Membrane-Protein Stability
| Content Provider | Scilit |
|---|---|
| Author | Hussain, Hazrat Du, Yang Tikhonova, Elena Mortensen, Jonas S. Ribeiro, Orquidea Santillan, Claudia Das, Manabendra Ehsan, Muhammad Loland, Claus J. Guan, Lan Kobilka, Brian K. Byrne, Bernadette Chae, Pil Seok |
| Copyright Year | 2017 |
| Description | Journal: Chemistry – A European Journal As a membrane-mimetic system, detergent micelles are popularly used to extract membrane proteins from lipid environments and to maintain their solubility and stability in an aqueous medium. However, many membrane proteins encapsulated in conventional detergents tend to undergo structural degradation during extraction and purification, thus necessitating the development of new agents with enhanced properties. In the current study, two classes of new amphiphiles are introduced, resorcinarene-based glucoside and maltoside amphiphiles (designated RGAs and RMAs, respectively), for which the alkyl chains are facially segregated from the carbohydrate head groups. Of these facial amphiphiles, two RGAs (RGA-C11 and RGA-C13) conferred markedly enhanced stability to four tested membrane proteins compared to a gold-standard conventional detergent. The relatively high water solubility and micellar stability of the RGAs compared to the RMAs, along with their generally favourable behaviours for membrane protein stabilisation described here, are likely to be, at least in part, a result of the high conformational flexibility of these glucosides. This study suggests that flexibility could be an important factor in determining the suitability of new detergents for membrane protein studies. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818266/pdf |
| Ending Page | 6729 |
| Page Count | 6 |
| Starting Page | 6724 |
| e-ISSN | 15213765 |
| DOI | 10.1002/chem.201605016 |
| Journal | Chemistry – A European Journal |
| Issue Number | 28 |
| Volume Number | 23 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2017-04-20 |
| Access Restriction | Open |
| Subject Keyword | Journal: Chemistry – A European Journal Facial Amphiphiles Membrane Proteins Molecular Design Protein Stability Resorcinarene Glycosides |
| Content Type | Text |
| Resource Type | Article |
