Nucleotide sequence and expression of the Pseudomonas aeruginosa algF gene controlling acetylation of alginate

Content Provider	Scilit
Author	Shinabarger, Dean May, Thomas B. Boyd, Aoife Ghosh, Mita Chakrabarty, A. M.
Copyright Year	1993
Description	Journal: Molecular microbiology Colonization of the cystic fibrosis lung by Pseudomonas aeruginosa is greatly facilitated by the production of an exopolysaccharide called alginate. In this study we determined the nucleotide sequence of an alginate modification gene, algF, which controls the addition of acetyl groups to alginate. Expression of algF using a T7 promoter‐expression system showed that algF codes for a 24.5 kDa polypeptide (predicted size 22 832 Da) that is processed to 19.5 kDa. The N‐terminus of the processed polypeptide matched the predicted amino acid sequence of AlgF starting at Asp‐29. An algF mutant failed to produce alginate owing to a polar effect on the downstream algA gene. Although the algA gene, provided in trans, restored synthesis of alginate, the alginate was non‐acetylated. We show that a plasmid containing both the algF and algA gene complements the alginate acetylation defect of the algF mutant strain.
Ending Page	1035
Starting Page	1027
ISSN	2573508X
e-ISSN	13652958
DOI	10.1111/j.1365-2958.1993.tb01232.x
Journal	Molecular microbiology
Issue Number	5
Volume Number	9
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1993-09-01
Access Restriction	Open
Subject Keyword	Journal: Molecular microbiology Biotechnology and Microbiology
Content Type	Text
Resource Type	Article