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Pre-synaptic $GABA_{B}$receptors inhibit glutamate release through GIRK channels in rat cerebral cortex
| Content Provider | Scilit |
|---|---|
| Author | Ladera, Carolina Godino, María Del Carmen Cabañero, María José Torres, Magdalena Watanabe, Masahiko Luján, Rafael Sánchez-Prieto, José |
| Copyright Year | 2008 |
| Description | Journal: Journal of Neurochemistry Neuronal G protein-gated inwardly rectifying potassium (GIRK) channels mediate the slow inhibitory effects of many neurotransmitters post-synaptically. However, no evidence exists that supports that GIRK channels play any role in the inhibition of glutamate release by $GABA_{B}$ receptors. In this study, we show for the first time that $GABA_{B}$ receptors operate through two mechanisms in nerve terminals from the cerebral cortex. As shown previously, $GABA_{B}$ receptors reduces glutamate release and the $Ca^{2+}$ influx mediated by N-type $Ca^{2+}$ channels in a mode insensitive to the GIRK channel blocker tertiapin-Q and consistent with direct inhibition of this voltage-gated $Ca^{2+}$ channel. However, by means of weak stimulation protocols, we reveal that $GABA_{B}$ receptors also reduce glutamate release mediated by P/Q-type $Ca^{2+}$ channels, and that these responses are reversed by the GIRK channel blocker tertiapin-Q. Consistent with the functional interaction between $GABA_{B}$ receptors and GIRK channels at nerve terminals we demonstrate by immunogold electron immunohistochemistry that pre-synaptic boutons of asymmetric synapses co-express $GABA_{B}$ receptors and GIRK channels, thus suggesting that the functional interaction of these two proteins, found at the post-synaptic level, also occurs at glutamatergic nerve terminals. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-4159.2008.05712.x |
| Ending Page | 1517 |
| Page Count | 12 |
| Starting Page | 1506 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.2008.05712.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 107 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2008-11-24 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Cerebrocortical Nerve Terminals Glutamate Release |
| Content Type | Text |
| Resource Type | Article |
