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In situ detection of hepatitis C virus RNA in liver tissue using a digoxigenin-labeled probe created during a polymerase chain reaction
| Content Provider | Scilit |
|---|---|
| Author | Cho, Sung Won Hwang, Seong Gyu Han, Dong Cheol Jin, So Young Lee, Moon Sung Shim, Chan Sup Lee, Dong Wha Lee, Hi Bahl |
| Copyright Year | 1996 |
| Description | Journal: Journal of Medical Virology The cellular localization of hepatitis C virus (HCV) RNA in liver tissue was studied by nonisotopic in situ hybridization using a digoxigenin-labeled cDNA probe created during a polymerase chain reaction on samples from 16 patients with chronic HCV infection. Hybridization signals were recognized in the cytoplasm of the hepatocytes, and a few hepatocytes had hybridization signals in the nucleus as well. HCV RNA positive hepatocytes were found in 1 of 9 patients with chronic persistent hepatitis, 2 of 5 patients with chronic active hepatitis, and in each of 2 patients with chronic active hepatitis and cirrhosis. Positive signals were found in many hepatocytes within the lobule in liver sections of patients with advanced chronic active hepatitis. A number of HCV RNA positive hepatocytes were found in nodules, but not in the area of fibrosis. On the other hand, positive signals were found in a few hepatocytes scattered in the lobule in a patient with chronic persistent hepatitis. The mean ALT levels in the patients with positive signal (175.6 ± 44.2 U/L) were significantly higher than in those without a signal (70.27 ± 16.1 U/L) (P < 0.05). The findings suggest that a larger amount of HCV may be present during the advanced than during the early stages of type C hepatitis and nonisotopic in situ hybridization using a digoxigenin-labeled HCV cDNA probe created during a polymerase chain reaction deserves wider application for the detection of HCV replication in specimens. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1002/(SICI)1096-9071(199603)48:3<227::AID-JMV3>3.0.CO;2-A |
| Ending Page | 233 |
| Page Count | 7 |
| Starting Page | 227 |
| e-ISSN | 10969071 |
| DOI | 10.1002/%28sici%291096-9071%28199603%2948%3A3%3C227%3A%3Aaid-jmv3%3E3.0.co%3B2-a |
| Journal | Journal of Medical Virology |
| Issue Number | 3 |
| Volume Number | 48 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1996-03-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Medical Virology Chronic Hepatitis C in Situ Hybridization Digoxigenin Labeling Polymerase Chain Reaction |
| Content Type | Text |
| Resource Type | Article |
