Mutation Spectrum Induced by Singlet Oxygen in Escherichia coli Deficient in Exonuclease III

Content Provider	Scilit
Author	Agnez-Lima, Lucymara F. Mascio, Paolo Di Napolitano, Rita L. Fuchs, Robert P. Menck, Carlos F. M.
Copyright Year	1999
Description	Journal: Photochemistry and Photobiology The repair of singlet oxygen (1O2)-induced DNA lesions requires several enzymes of the nucleotide and base excision repair pathways, including exonuclease III and endonuclease IV that are known apurinic/apyrimidinic-endonucleases in Escherichia coli. In order to better understand the relevance of exonuclease III on the repair of these lesions, we investigated the mutagenic events that result from the replication of a 1O2-damaged plasmid in an exonuclease-deficient host (xth). The mutation spectrum in the tRNA supF gene target indicated that the absence of exonuclease III does not change the types of mutations induced by 1O2 (mostly of G:C-->T:A and G:C-->C:G transversions). However, the spectrum shows that the mutations are scattered in the supF gene, which is significatively different from the one obtained in wild-type bacteria. Thus, exonuclease III may act on the repair of 1O2-induced lesions altering the DNA repair sequence specificity.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1751-1097.1999.tb08245.x/pdf
Ending Page	511
Page Count	7
Starting Page	505
e-ISSN	17511097
DOI	10.1562/0031-8655%281999%29070%3C0505%3Amsibso%3E2.3.co%3B2
Journal	Photochemistry and Photobiology
Issue Number	4
Volume Number	70
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1999-01-01
Access Restriction	Open
Subject Keyword	Journal: Photochemistry and Photobiology Biochemistry and Molecular Biology
Content Type	Text
Resource Type	Article