NDLI: NADP-Dependent Isocitrate Dehydrogenase from the Mussel Mytilus edulis L.. 1. Purification and Characterisation

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NADP-Dependent Isocitrate Dehydrogenase from the Mussel Mytilus edulis L.. 1. Purification and Characterisation

Content Provider	Scilit
Author	Head, Erica J. H.
Copyright Year	1980
Description	Journal: Journal of Biological Inorganic Chemistry A purification scheme is described for NADP-dependent isocitrate dehydrogenase from the digestive gland of Mytilus edulis. The scheme incorporates three chromatographic steps: hydroxyapatite adsorption, blue-Sepharose affinity chromatography and DEAE-Sephacel ion-exchange chromatography. The subunit molecular weight of the enzyme was 45 000 (+/- 5000) by sodium dodecylsulfate gel electrophoresis. The purified enzyme was homogeneous on dodecylsulfate gels and had a specific activity of 30--50 U/mg protein.
Related Links	http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1980.tb04973.x/pdf
Ending Page	579
Page Count	5
Starting Page	575
ISSN	09498257
e-ISSN	14321327
DOI	10.1111/j.1432-1033.1980.tb04973.x
Journal	Journal of Biological Inorganic Chemistry
Issue Number	2
Volume Number	111
Language	English
Publisher	Wiley-Blackwell
Publisher Date	1980-10-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Inorganic Chemistry Biochemical Research Isocitrate Dehydrogenase Dependent Isocitrate
Content Type	Text
Resource Type	Article
Subject	Biochemistry Inorganic Chemistry

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