Loading...
Please wait, while we are loading the content...
Similar Documents
Ca2+and Calmodulin-Dependent Phosphorylation of Endogenous Synaptic Vesicle Tubulin by a Vesicle-Bound Calmodulin Kinase System
| Content Provider | Scilit |
|---|---|
| Author | Burke, Briant E. DeLorenzo, Robert J. |
| Copyright Year | 1982 |
| Description | Journal: Journal of Neurochemistry Endogenous synaptic vesicle alpha- and beta-tubulin were shown to be the major substrates for a Ca2+-calmodulin-regulated protein kinase system in enriched synaptic vesicle preparations from rat cortex as determined by two-dimensional gel electrophoresis and peptide mapping. The activation of this endogenous tubulin kinase system was dependent on Ca2+ and the Ca2+ binding protein, calmodulin. Under maximally stimulated conditions, approximately 40% of the tubulin present in enriched synaptic vesicles was phosphorylated within less than 50 s by the vesicle Ca2+-calmodulin kinase. Evidence is presented indicating that the Ca2+-calmodulin tubulin kinase is an enzyme system distinct from previously described cyclic AMP protein kinases. alpha-Tubulin and beta-tubulin were identified as major components of previously designated vesicle phosphorylation bands DPH-L and DPH-M. The Ca2+-calmodulin tubulin kinase is very labile and specialized isolation procedures were necessary to retain activity. Ca2+-activated synaptic vesicle tubulin phosphorylation correlated with vesicle neurotransmitter release. Depolarization-dependent Ca2+ uptake in intact synaptosomes simultaneously stimulated the release of neurotransmitters and the phosphorylation of synaptic vesicle alpha- and beta-tubulin. The results indicate that regulation of the synaptic vesicle tubulin kinase by Ca2+ and calmodulin may play a role in the functional utilization of synaptic vesicle tubulin and may mediate some of the effects of Ca2+ on vesicle function and neurosecretion. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1982.tb07892.x/pdf |
| Ending Page | 1218 |
| Page Count | 14 |
| Starting Page | 1205 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1982.tb07892.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 5 |
| Volume Number | 38 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1982-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Neurotransmitter Release Protein Phosphorylation |
| Content Type | Text |
| Resource Type | Article |
