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Molecular cloning and expression of Galbeta1,3GalNAcalpha2,3-sialyltransferase from mouse brain
| Content Provider | Scilit |
|---|---|
| Author | Lee, Young-Choon Kurosawa, Nobuyuki Hamamoto, Toshiro Nakaoka, Takashi Tsuji, Shuichi |
| Copyright Year | 1993 |
| Description | Journal: Journal of Biological Inorganic Chemistry DNA clones encoding β-galactoside α2,3-sialyltransferase have been isolated from mouse brain cDNA libraries using sequence information obtained from the conserved amino acid sequence of the previously cloned enzymes. The cDNA sequence revealed an open reading frame coding for 337 amino acids, and the deduced amono acid sequence showed 80% identity with that of porcine submaxillary gland Galβ1,3GalNAc α2,3-sialyltransferase. The primary structure of this enzyme suggested a putative domain structure, like that in other glycosyltransferases, consisting of a short $NH_{2}$-terminal cytoplasmic domain, a signal-membrane anchor domain, a proteolytically sensitive stem region, and a large COOH-terminal active domain. The identity of this enzyme was confirmed by construction of a recombinant sialyltransferase in which the $NH_{2}$-terminal part including the cytoplasmic tail, signal-anchor domain and stem region was replaced with an immuno-globulin signal sequence. The expression of this recombinant in COS-7 cells resulted in secretion of a catalytically active and soluble form of the enzyme into the medium. This enzyme exhibited the transferase activity toward only the disaccharide moiety of Galβ1,3GalNAc of glycoproteins and glycolipids, no significant activity being detected for the other substrates tested. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1993.tb18155.x |
| Ending Page | 385 |
| Page Count | 9 |
| Starting Page | 377 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1993.tb18155.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 216 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1993-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biotechnology and Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
