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Structural and functional studies of the interaction of the eukaryotic elongation factor EF-2 with GTP and ribosomes
| Content Provider | Scilit |
|---|---|
| Author | Nilsson, Lars Nygard, Odd |
| Copyright Year | 1988 |
| Description | Journal: Journal of Biological Inorganic Chemistry The structure of the guanosine nucleotide binding site of EF-2 was studied by affinity labelling with the GTP analogue, oxidized GTP (oGTP), and by amino acid sequencing of polypeptides generated after partial degradation with trypsin and N-chlorosuccinimide. Native EF-2 contains two exposed trypsin-sensitive cleavage sites. One site is at Arg66 with a second site at Lys571/Lys572. oGTP was covalently bound to the factor between Arg66 and Lys571. After further cleavage of this fragment with the tryptophan-specific cleavage reagent N-chlorosuccinimide, oGTP was found associated with a polypeptide fragment originating from a cleavage at Trp261 and Trp343. The covalent oGTP . EF-2 complex was capable of forming a high-affinity complex with ribosomes, indicating that oGTP, in this respect, induced a conformation in EF-2 indistinguishable from that produced by GTP. Although GTP could be substituted by non-covalently linked oGTP in the factor and ribosome-dependent GTPase reaction, the factor was unable to utilize the covalently bound oGTP as a substrate. This indicates that the conformational flexibility in EF-2 required for the ribosomal activation of the GTPase was inhibited by the covalent attachment of the nucleotide to the factor. EF-2 cleaved at Arg66 were unable to form the high-affinity complex with ribosomes while retaining the ability to form the low-affinity complex and to hydrolyse GTP. The second cleavage at Lys571/Lys572 was accompanied by a total loss of both the low-affinity binding and the GTPase activity. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1988.tb13789.x/pdf |
| Ending Page | 299 |
| Page Count | 7 |
| Starting Page | 293 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1988.tb13789.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1-2 |
| Volume Number | 171 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1988-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
