NDLI: Loop‐mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens

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Loop‐mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens

Content Provider	Scilit
Author	Savan, R. Kono, T. Itami, T. Sakai, M.
Copyright Year	2005
Description	Journal: Journal of Fish Diseases Fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. Early diagnosis plays a vital role in management of fish and shellfish diseases. Traditionally, various biochemical and serological tests have been used for fish disease diagnosis. However, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. Polymerase chain reaction and probe‐based nucleic acid detection have become increasingly popular in fish and shellfish diagnostics. Recently, a novel technique called loop‐mediated isothermal amplification (LAMP) has been developed, which is highly sensitive and rapid. LAMP has been used for the detection of bacterial, viral, fungal and parasitic diseases in both animal and plants. In aquaculture, LAMP‐based detection of pathogens like Edwardsiella tarda, E. ictaluri, Nocardia seriolae, Tetracapsuloides bryosalmonae, white spot syndrome virus and infectious haematopoietic necrosis virus have been reported. In this review, the application of LAMP for the detection of aquaculture‐associated pathogens is discussed.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197467/pdf
Ending Page	581
Page Count	9
Starting Page	573
e-ISSN	13652761
DOI	10.1111/j.1365-2761.2005.00670.x
Journal	Journal of Fish Diseases
Issue Number	10
Volume Number	28
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2005-10-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Fish Diseases Fisheries Sciences Diagnostic Method Loop‐mediated Isothermal Amplification
Content Type	Text
Resource Type	Article

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