NDLI: Real-Time Monitoring of New Delhi Metallo-β-Lactamase Activity in Living Bacterial Cells $by^{1}$H NMR Spectroscopy

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Real-Time Monitoring of New Delhi Metallo-β-Lactamase Activity in Living Bacterial Cells $by^{1}$H NMR Spectroscopy

Content Provider	Scilit
Author	Ma, Junhe McLeod, Sarah Cormack, Kathleen Mac Sriram, Shubha Gao, Ning Breeze, Alexander L. Hu, Jun
Copyright Year	2014
Description	Journal: Angewandte Chemie International Edition Disconnections between in vitro responses and those observed in whole cells confound many attempts to design drugs in areas of serious medical need. A method based on 1D$ ^{1}$H NMR spectroscopy is reported that affords the ability to monitor the hydrolytic decomposition of the carbapenem antibiotic meropenem inside Escherichia coli cells expressing New Delhi metallo‐β‐lactamase subclass 1 (NDM‐1), an emerging antibiotic‐resistance threat. Cell‐based NMR studies demonstrated that two known NDM‐1 inhibitors, L‐captopril and ethylenediaminetetraacetic acid (EDTA), inhibit the hydrolysis of meropenem in vivo. NDM‐1 activity in cells was also shown to be inhibited by spermine, a porin inhibitor, although in an in vitro assay, the influence of spermine on the activity of isolated NDM‐1 protein is minimal. This new approach may have generic utility for monitoring reactions involving diffusible metabolites in other complex biological matrices and whole‐cell settings, including mammalian cells.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232273/pdf
Ending Page	2133
Page Count	4
Starting Page	2130
ISSN	14337851
e-ISSN	15213773
DOI	10.1002/anie.201308636
Journal	Angewandte Chemie International Edition
Issue Number	8
Volume Number	53
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2014-01-23
Access Restriction	Open
Subject Keyword	Journal: Angewandte Chemie International Edition Pharmacology and Pharmacy Antibiotic Resistance Drug Discovery New Delhi Metallo‐β‐lactamase Nmr Spectroscopy
Content Type	Text
Resource Type	Article
Subject	Chemistry Catalysis

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