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Biosynthesis of Sterols by a Yeast Homogenate. Incorporation of Mevalonic Acid into Cholesta-5,7,24-trien-3beta-ol and 5alpha-Cholesta-7,24-dien-3beta-ol
| Content Provider | Scilit |
|---|---|
| Author | Moreau, Jacques P. Caspi, Eliahu Ramm, Peter J. |
| Copyright Year | 1975 |
| Description | Journal: Journal of Biological Inorganic Chemistry Incubation of (3RS,2R)-[2-14C,2-3H]mevalonic acid and (3RS, 2S)-[2-14C,2-3H]mevalonic acid with mechanically disrupted yeast cells resulted in C27-metabolites. Two (14C5, 3H4)-metabolites, cholesta-5,7,24-trien-3beta-ol and 5 alpha-cholesta-7,24-dien-3beta-ol, were isolated and characterized. The impairment of the 24-methyl transferase system was confirmed by the lack of incorporation of 14C into the sterol fraction on incubation of S-adenosyl-L-[methyl-14C]methionine with the yeast homogenate. The results indicate that interference with the (C-24)-alkylating system did not prevent the transformation of lanosterol to the cholesta-5,7,24-trien-3beta-ol and to 5 alpha-cholesta-7,24-dien-3beta-ol. It can therefore be inferred that transformations of the nucleus and of the side chain can function independently. However our results do not provide a definition of the actual sequence of the metabolic events between lanosterol and ergosterol. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1975.tb02245.x/pdf |
| Ending Page | 402 |
| Page Count | 10 |
| Starting Page | 393 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1975.tb02245.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 56 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1975-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Trien 3beta Ol Dien 3beta Ol |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
