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Land-use systems affect Archaeal community structure and functional diversity in western Amazon soils
| Content Provider | Scilit |
|---|---|
| Author | Navarrete, Acácio Aparecido Taketani, Rodrigo Gouvêa Mendes, Lucas William Cannavan, Fabiana De Souza Moreira, Fatima Maria De Souza Tsai, Siu Mui |
| Copyright Year | 2011 |
| Description | The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the 16S rRNA gene as a biomarker showed that archaeal community structures in crops and pasture soils are different from those in primary forest soil, which is more similar to the community structure in secondary forest soil. Sequence analysis of excised DGGE bands indicated the presence of crenarchaeal and euryarchaeal organisms. Based on clone library analysis of the gene coding the subunit of the enzyme ammonia monooxygenase (amoA) of Archaea (306 sequences), the Shannon-Wiener function and Simpson's index showed a greater ammonia-oxidizing archaeal diversity in primary forest soils (H' = 2.1486; D = 0.1366), followed by a lower diversity in soils under pasture (H' = 1.9629; D = 0.1715), crops (H' = 1.4613; D = 0.3309) and secondary forest (H' = 0.8633; D = 0.5405). All cloned inserts were similar to the Crenarchaeota amoA gene clones (identity > 95 %) previously found in soils and sediments and distributed primarily in three major phylogenetic clusters. The findings indicate that agricultural systems of indigenous people and cattle pasture affect the archaeal community structure and diversity of ammonia-oxidizing Archaea in western Amazon soils. |
| Related Links | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-06832011000500007&lng=en&nrm=iso&tlng=en |
| Ending Page | 1540 |
| Page Count | 14 |
| Starting Page | 1527 |
| e-ISSN | 01000683 |
| DOI | 10.1590/s0100-06832011000500007 |
| Journal | Revista Brasileira de Ciência Do Solo |
| Issue Number | 5 |
| Volume Number | 35 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2011-10-01 |
| Access Restriction | Open |
| Subject Keyword | Revista Brasileira de Ciência Do Solo Soil Science Soil Microbial Ecology Soil Microbiology Microbial Diversity Land Use Changes Tropical Soils Ecologia Microbiana Do Solo Microbiologia Do Solo Diversidade Microbiana Mudança De Uso Da Terra Solos Tropicais |
| Content Type | Text |
| Resource Type | Article |
