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Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
| Content Provider | Scilit |
|---|---|
| Author | Li, Jiang Hu, Qiushi Li, Yuquan Xu, Yuan |
| Copyright Year | 2015 |
| Description | An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of $Mg^{2+}$, $Mn^{2+}$, $K^{+}$; $Ca^{2+}$, $Na^{+}$, $Ba^{2+}$, $Zn^{2+}$, $Cu^{2+}$, $Co^{2+}$, $Fe^{2+}$, $Sr^{2+}$ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4568857/pdf http://www.scielo.br/pdf/bjm/v46n3/1678-4405-bjm-46-20131289.pdf |
| Ending Page | 690 |
| Page Count | 8 |
| Starting Page | 683 |
| ISSN | 15178382 |
| e-ISSN | 16784405 |
| DOI | 10.1590/s1517-838246320131289 |
| Journal | Brazilian Journal of Microbiology |
| Issue Number | 3 |
| Volume Number | 46 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2015-09-01 |
| Access Restriction | Open |
| Subject Keyword | Brazilian Journal of Microbiology Biotechnology and Microbiology adapted Agarase Psychrophilic Bacteria |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
