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Imobilização de enzimas a partir de "kit" comercial: determinação de parâmetros metabólicos em sangue animal empregando multicomutação em fluxo
| Content Provider | Scilit |
|---|---|
| Author | Pires, Cherrine Kelce Reis, Boaventura Freire dos |
| Copyright Year | 2005 |
| Description | Automatic flow procedures based on the multicommutation concept, dedicated to the determination of 3-hydroxybutyrate, glucose and cholesterol are proposed. The enzymes were immobilized on glass beads and packed into mini-columns that were coupled to a flow system. Sampling throughputs of 55, 40 and 40 determinations per hour, linear response from 10 to 150, 50 to 600, 25 to 125 mg L-1, detection limits of 1.5, 14 and 4 mg L-1 and relative standard deviations of 1, 2 and 2% for 3-hydroxybutyrate, glucose and cholesterol, respectively, were achieved |
| Related Links | http://www.scielo.br/pdf/qn/v28n3/24129.pdf http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-40422005000300010&lng=pt&nrm=iso&tlng=pt |
| Ending Page | 420 |
| Page Count | 7 |
| Starting Page | 414 |
| ISSN | 01004042 |
| e-ISSN | 01004042 |
| DOI | 10.1590/s0100-40422005000300010 |
| Journal | Química Nova |
| Issue Number | 3 |
| Volume Number | 28 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2005-06-01 |
| Access Restriction | Open |
| Subject Keyword | Química Nova Empregando Multicomutao Parmetros Metablicos Animal Empregando |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry |