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Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
| Content Provider | Scilit |
|---|---|
| Author | De-Paris, Fernanda Machado, Alice Beatriz Mombach Pinheiro Gheno, Tailise Conte Ascoli, Bruna Maria de Oliveira, Kátia Ruschel Pilger Barth, Afonso Luis |
| Copyright Year | 2011 |
| Description | Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection |
| Related Links | http://www.scielo.br/pdf/bjid/v15n4/v15n4a04.pdf http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1413-86702011000400004&lng=en&nrm=iso&tlng=en |
| Ending Page | 327 |
| Page Count | 5 |
| Starting Page | 323 |
| ISSN | 14138670 |
| e-ISSN | 16784391 |
| DOI | 10.1590/s1413-86702011000400004 |
| Journal | The Brazilian Journal of Infectious Diseases |
| Issue Number | 4 |
| Volume Number | 15 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2011-08-01 |
| Access Restriction | Open |
| Subject Keyword | The Brazilian Journal of Infectious Diseases Medical Laboratory Technology Streptococcus Agalactiae Polymerase Chain Reaction |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Microbiology (medical) |
