Determinação indireta de N-acetil-L-cisteína por injeção em fluxo empregando Ce(IV) e ferroína

Content Provider	Scilit
Author	Vieira, Heberth Juliano Fatibello-Filho, Orlando
Copyright Year	2005
Description	An indirect flow injection spectrophotometric procedure is proposed for the determination of N-acetyl-L-cysteine in pharmaceutical formulations. In this system, ferroin ([Fe(II)-(fen)2]2+) in excess, with a strong absorption at 500 nm, is oxidized by cerium(IV) yielding cerium(III) and [Fe(III)-(fen)2]3+ (colorless), thus producing a baseline. When N-acetyl-L-cysteine solution is introduced into the flow injection system, it reacts with cerium(IV) increasing the analytical signal in proportion to the drug concentration. Under optimal experimental conditions, the linearity of the analytical curve for N-acetyl-L-cysteine ranged from 6.5x10-6 to 1.3x10-4 mol L-1. The detection limit was 5.0x10-6 mol L-1and recoveries between 98.0 and 106% were obtained. The sampling frequency was 60 determinations per hour and the RSD was smaller than 1.4% for 2.2x10-5 mol L-1 N-acetyl-L-cysteine
Related Links	http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-40422005000500012&lng=pt&nrm=iso&tlng=pt
Ending Page	800
Page Count	4
Starting Page	797
ISSN	01004042
e-ISSN	01004042
DOI	10.1590/s0100-40422005000500012
Journal	Química Nova
Issue Number	5
Volume Number	28
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2005-10-01
Access Restriction	Open
Subject Keyword	Química Nova Medical Laboratory Technology Determinao Indireta
Content Type	Text
Resource Type	Article
Subject	Chemistry