NDLI: Cloning and expression of meta-cleavage enzyme (CarB) of carbazole degradation pathway from Pseudomonas stutzeri

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Cloning and expression of meta-cleavage enzyme (CarB) of carbazole degradation pathway from Pseudomonas stutzeri

Content Provider	Scilit
Author	Larentis, Ariane Leites Alves, Tito Lívio Moitinho Martins, Orlando Bonifácio
Copyright Year	2005
Description	In this work, the 1082bp PCR product corresponding to carBaBb genes that encode the heterotetrameric enzyme 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase (CarB), involved in the Pseudomonas stutzeri ATCC 31258 carbazole degradation pathway, was cloned using the site-specific recombination system. Recombinant clones were confirmed by PCR, restriction enzyme digestion and sequencing. CarB dioxygenase was expressed in high levels and in active form in Escherichia coli BL21-SI using the His-tagged expression vector pDEST TM17 and salt induction for 4h.
Related Links	http://www.scielo.br/pdf/babt/v48nspe/25287.pdf
Ending Page	134
Page Count	8
Starting Page	127
ISSN	15168913
e-ISSN	16784324
DOI	10.1590/s1516-89132005000400016
Journal	Brazilian Archives of Biology and Technology
Issue Number	spe
Volume Number	48
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2005-06-01
Access Restriction	Open
Subject Keyword	Brazilian Archives of Biology and Technology Biochemistry and Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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