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Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure
| Content Provider | Scilit |
|---|---|
| Author | Gonçalves, Frances Lilian Lanhellas Gallindo, Rodrigo Melo Soares, Lucas Manoel Mangueira Figueira, Rebeca Volpe, Fábio Antônio Perecim Pereira-Da-Silva, Maurício André Sbragia, Lourenço |
| Copyright Year | 2013 |
| Description | PURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac®, PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p |
| Related Links | http://www.scielo.br/pdf/acb/v28s1/v28s1a05.pdf |
| Ending Page | 25 |
| Page Count | 7 |
| Starting Page | 19 |
| ISSN | 01028650 |
| e-ISSN | 16782674 |
| DOI | 10.1590/s0102-86502013001300005 |
| Journal | Acta Cirurgica Brasileira |
| Issue Number | suppl 1 |
| Volume Number | 28 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2013-01-01 |
| Access Restriction | Open |
| Subject Keyword | Acta Cirurgica Brasileira Pediatrics and Child Health |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surgery |
