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Use of chromosome microdissection in fish molecular cytogenetics
| Content Provider | Scilit |
|---|---|
| Author | Henning, Frederico Trifonov, Vladimir de Almeida-Toledo, Lurdes Foresti |
| Copyright Year | 2008 |
| Description | Chromosome microdissection is a technique in which whole chromosomes or chromosomal segments are dissected under an inverted microscope yielding chromosome-specific sequences. Several protocol modifications introduced during the past 15 years reduced the number of chromosomes required for most applications. This is of particular interest to fish molecular cytogenetics, since most species present highly uniform karyotypes which make impossible the collection of multiple copies of the same chromosome. Probes developed in this manner can be used to investigate chromosome homologies in closely related species. Here we describe a protocol recently used in the gymnotiform species group Eigenmannia and review the major steps involved in the generation of these markers focusing on protocol modifications aiming to reduce the number of required chromosomes. |
| Related Links | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S1415-47572008000200022&lng=en&nrm=iso&tlng=en |
| Ending Page | 283 |
| Page Count | 5 |
| Starting Page | 279 |
| ISSN | 14154757 |
| e-ISSN | 16784685 |
| DOI | 10.1590/s1415-47572008000200022 |
| Journal | Genetics and Molecular Biology |
| Issue Number | 1 |
| Volume Number | 31 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Subject Keyword | Genetics and Molecular Biology Biochemical Research Fluorescence in Situ Hybridization Chromosome Painting |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Molecular Biology |
