NDLI: Efficient mispriming during apolipoprotein E genotyping

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Efficient mispriming during apolipoprotein E genotyping

Content Provider	Scilit
Author	de Azevedo Souza, Gabriela Merheb Romano, Nara Lúcia Whittle, Martin R.
Copyright Year	2005
Description	The determination of ApoE genotypes is a common clinical laboratory procedure. The most frequently used method involves a PCR amplification of the target region followed by a restriction enzyme digestion of the resultant amplicon to obtain a genotype-specific restriction fragment pattern. We describe an unexpected and surprising difficulty that was encountered during our routine laboratory casework. A determinação do genótipo da apolipoproteína E (ApoE) é um procedimento clinicolaboratorial comum. O método mais freqüentemente utilizado envolve a amplificação por reação em cadeia de polimerase (PCR) de uma região alvo, seguida pela digestão com enzima de restrição do fragmento resultante, obtendo-se, assim, um modelo de fragmentos de restrição genótipo-específico. Nós descrevemos uma dificuldade inesperada que encontramos durante nossa rotina laboratorial
Related Links	http://www.scielo.br/pdf/jbpml/v41n1/a07v41n1.pdf
ISSN	16762444
e-ISSN	16784774
DOI	10.1590/s1676-24442005000100007
Journal	Jornal Brasileiro de Patologia E Medicina Laboratorial
Issue Number	1
Volume Number	41
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2005-02-01
Access Restriction	Open
Subject Keyword	Jornal Brasileiro de Patologia E Medicina Laboratorial Medical Laboratory Technology Polymerase Chain Reaction Apolipoprotein E Apoe Genotyping Reação Em Cadeia De Polimerase Apolipoproteína E Genotipagem De Apoe
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Medical Laboratory Technology Pathology and Forensic Medicine

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