Characterization of Biomphalaria orbignyi, Biomphalaria peregrina and Biomphalaria oligoza by polymerase chain reaction and restriction enzyme digestion of the internal transcribed spacer region of the RNA ribosomal gene.

Content Provider	Scilit
Author	Spatz, Linus Vidigal, Teofânia Hda Silva, Márcia Ca Cappa, Stella Maris Gonzalez Carvalho, Omar S.
Copyright Year	2000
Description	The correct identification of Biomphalaria oligoza, B. orbignyi and B. peregrina species is difficult due to the morphological similarities among them. B. peregrina is widely distributed in South America and is considered a potential intermediate host of Schistosoma mansoni. We have reported the use of the polymerase chain reaction and restriction fragment length polymorphism analysis of the internal transcribed spacer region of the ribosomal DNA for the molecular identification of these snails. The snails were obtained from different localities of Argentina, Brazil and Uruguay. The restriction patterns obtained with MvaI enzyme presented the best profile to identify the three species. The profiles obtained with all enzymes were used to estimate genetic similarities among B. oligoza, B. peregrina and B. orbignyi. This is also the first report of B. orbignyi in Uruguay.
Related Links	http://www.scielo.br/pdf/mioc/v95n6/v95n6a10.pdf http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0074-02762000000600010&lng=en&nrm=iso&tlng=en
Ending Page	814
Page Count	8
Starting Page	807
ISSN	00740276
e-ISSN	16788060
DOI	10.1590/s0074-02762000000600010
Journal	Memorias do Instituto Oswaldo Cruz
Issue Number	6
Volume Number	95
Language	English
Publisher	FapUNIFESP (SciELO)
Publisher Date	2000-12-01
Access Restriction	Open
Subject Keyword	Memorias do Instituto Oswaldo Cruz Biomphalaria Orbignyi Biomphalaria Peregrina Biomphalaria Oligoza Internal Transcribed Spacer Polymerase Chain Reaction
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)