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Determinação da 2,5-hexanodiona em amostras de urina submetidas à hidrólise ácida: importância da coluna cromatográfica e do pH do meio
| Content Provider | Scilit |
|---|---|
| Author | Barroca, Márcia Martins Silveira, Josianne Nicácio Alvarez-Leite, Edna Maria |
| Copyright Year | 2003 |
| Description | Acidic hydrolysis of samples is frequent in urinary 2,5-hexanodione determination. This hydrolysis should be performed under proper conditions, in order to avoid interference, such as the presence of 2,5-dimethylfurane and 2-acetylfurane. The results of the present work, as well as data from the literature, show that the use of non-polar or slightly polar chromatographic columns of 30 m length is an essential condition for 2,5-HD determination in an acid hydrolysed urine sample. In the same way the pH should be kept between 0.3 and 0.5. The mean concentrations of 2,5-HD in samples submitted to hydrolysis (0.50 ± 0.28 mg/g of creatinine) was about 3 times higher than those found in the samples without acid hydrolysis (0.19 ± 0.3 mg/g of creatinine) |
| Related Links | http://www.scielo.br/scielo.php?script=sci_pdf&pid=S0100-40422003000400021&lng=pt&nrm=iso&tlng=pt |
| Ending Page | 572 |
| Page Count | 3 |
| Starting Page | 570 |
| ISSN | 01004042 |
| e-ISSN | 01004042 |
| DOI | 10.1590/s0100-40422003000400021 |
| Journal | Química Nova |
| Issue Number | 4 |
| Volume Number | 26 |
| Language | English |
| Publisher | FapUNIFESP (SciELO) |
| Publisher Date | 2003-08-01 |
| Access Restriction | Open |
| Subject Keyword | Química Nova |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry |
