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Regulated viral BDNF delivery in combination with Schwann cells promotes axonal regeneration through capillary alginate hydrogels after spinal cord injury
| Content Provider | Scilit |
|---|---|
| Author | LaShae, Nicholson Liu, Shengwen Sandner, Beatrice Schackel, Thomas Chtarto, Abdelwahed Tenenbaum, Liliane Puttagunta, Radhika Müller, Rainer Weidner, Norbert Blesch, Armin |
| Copyright Year | 2017 |
| Description | Journal: Acta Biomaterialia Grafting of cell-seeded alginate capillary hydrogels into a spinal cord lesion site provides an axonal bridge while physically directing regenerating axonal growth in a linear pattern. However, without an additional growth stimulus, bridging axons fail to extend into the distal host spinal cord. Here we examined whether a combinatory strategy would support regeneration of descending axons across a cervical (C5) lateral hemisection lesion in the rat spinal cord. Following spinal cord transections, Schwann cell (SC)-seeded alginate hydrogels were grafted to the lesion site and AAV5 expressing brain-derived neurotrophic factor (BDNF) under control of a tetracycline-regulated promoter was injected caudally. In addition, we examined whether SC injection into the caudal spinal parenchyma would further enhance regeneration of descending axons to re-enter the host spinal cord. Our data show that both serotonergic and descending axons traced by biotinylated dextran amine (BDA) extend throughout the scaffolds. The number of regenerating axons is significantly increased when caudal BDNF expression is activated and transient BDNF delivery is able to sustain axons after gene expression is switched off. Descending axons are confined to the caudal graft/host interface even with continuous BDNF expression for 8 weeks. Only with a caudal injection of SCs, a pathway facilitating axonal regeneration through the host/graft interface is generated allowing axons to successfully re-enter the caudal spinal cord.Recovery from spinal cord injury is poor due to the limited regeneration observed in the adult mammalian central nervous system. Biomaterials, cell transplantation and growth factors that can guide axons across a lesion site, provide a cellular substrate, stimulate axon growth and have shown some promise in increasing the growth distance of regenerating axons. In the present study, we combined an alginate biomaterial with linear channels with transplantation of Schwann cells within and beyond the lesion site and injection of a regulatable vector for the transient expression of brain-derived neurotrophic factor (BDNF). Our data show that only with the full combination axons extend across the lesion site and that expression of BDNF beyond 4 weeks does not further increase the number of regenerating axons. |
| Related Links | https://scholarworks.iupui.edu/bitstream/1805/14058/1/Liu_2017_regulated.pdf |
| Ending Page | 180 |
| Page Count | 14 |
| Starting Page | 167 |
| ISSN | 17427061 |
| e-ISSN | 18787568 |
| DOI | 10.1016/j.actbio.2017.07.024 |
| Journal | Acta Biomaterialia |
| Volume Number | 60 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Acta Biomaterialia Alginate Hydrogel Spinal Cord Injury Brain-derived Neurotrophic Factor Axonal Regeneration Regulated Gene Expression |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Molecular Biology Biomaterials Biomedical Engineering Biotechnology |
