Overexpression of microRNA-30a contributes to the development of aortic dissection by targeting lysyl oxidase

Content Provider	Scilit
Author	Yu, Yang Shi, Enyi Gu, Tianxiang Tang, Rui Gao, Shilun Wang, Yongchao Liu, Hongbo
Copyright Year	2017
Description	Journal: Journal of Thoracic and Cardiovascular Surgery Objective To explore the role of microRNA (miR)-30a in the development of aortic dissection. Methods Human aortic specimens of aortic dissections and aneurysms were harvested. Aortic specimens from donors for heart transplantation served as controls. Rat aortic vascular smooth muscle cells (VSMCs) were transfected with agomiR-30a or antagomiR-30a, and control cells were incubated with empty vectors. Rats were pretreated with agomiR-30a or antagomiR-30a (5 × $10^{7}$ transfection units every 3 days for 4 weeks), and empty vectors were infused to controls. Acute aortic dissection was induced by subcutaneous infusion of angiotensin II (1 μg · $kg^{−1}$ · $min^{−1}$ for 24 hours). Protein expressions of lysyl oxidase (LOX) and elastin and gene expression of miR-30a were measured in VSMCs and human and rat aortic specimens by Western blot analysis and quantitative real-time polymerase chain reaction. Results Gene expression of miR-30a was much higher, and protein abundance of LOX and elastin was significantly lower, in the aortic dissection specimens (P < .05 vs controls). Transfection of agomiR-30a markedly decreased the luciferase activity of LOX in VSMCs of wild type, but not of LOX 3′-UTR mutant (P = .002). In cultured VSMCs, transfection of agomiR-30a dramatically enhanced the gene expression of miR-30a and down-regulated the protein abundance of LOX and elastin (P < .05 vs controls). Pretreatment with agomiR-30a in vivo enhanced miR-30a expression and down-regulated the protein abundance of LOX and elastin in rat aortas (P < .05 vs controls). The rate of dissection was significantly higher in rats pretreated with agomiR-30a (P = .003 vs controls). Conclusions Overexpression of miR-30a contributes to the development of aortic dissection, possibly by targeting LOX.
Related Links	http://www.jtcvs.org/article/S0022522317312291/pdf
Ending Page	1869
Page Count	8
Starting Page	1862
ISSN	00225223
e-ISSN	1097685X
DOI	10.1016/j.jtcvs.2017.06.019
Journal	Journal of Thoracic and Cardiovascular Surgery
Issue Number	6
Volume Number	154
Language	English
Publisher	Elsevier BV
Publisher Date	2017-06-16
Access Restriction	Open
Subject Keyword	Journal: Journal of Thoracic and Cardiovascular Surgery Gastroenterology and Hepatology Aortic Dissection Quantitative Real-time Polymerase Chain Reaction Vascular Smooth Muscle Cell
Content Type	Text
Resource Type	Article
Subject	Pulmonary and Respiratory Medicine Surgery Cardiology and Cardiovascular Medicine