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Lunapark Is a Component of a Ubiquitin Ligase Complex Localized to the Endoplasmic Reticulum Three-way Junctions
| Content Provider | Scilit |
|---|---|
| Author | Zhao, Yupeng Zhang, Ting Huo, Huanhuan Ye, Yihong Liu, Yanfen |
| Copyright Year | 2016 |
| Description | Journal: Journal of Biological Chemistry The endoplasmic reticulum (ER) network comprises sheets and tubules that are connected by dynamic three-way junctions. Lunapark (Lnp) localizes to and stabilizes ER three-way junctions by antagonizing the small GTPase Atlastin, but how Lnp shapes the ER network is unclear. Here, we used an affinity purification approach and mass spectrometry to identify Lnp as an interacting partner of the ER protein quality control ubiquitin ligase gp78. Accordingly, Lnp purified from mammalian cells has a ubiquitin ligase activity in vitro. Intriguingly, biochemical analyses show that this activity can be attributed not only to associated ubiquitin ligase, but also to an intrinsic ubiquitin ligase activity borne by Lnp itself. This activity is contained in the N-terminal 45 amino acids of Lnp although this segment does not share homology to any known ubiquitin ligase motifs. Despite its interaction with gp78, Lnp does not seem to have a broad function in degradation of misfolded ER proteins. On the other hand, the N-terminal ubiquitin ligase-bearing motif is required for the ER three-way junction localization of Lnp. Our study identifies a new type of ubiquitin ligase and reveals a potential link between ubiquitin and ER morphology regulation. |
| Related Links | http://www.jbc.org/content/291/35/18252.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000073/pdf http://www.jbc.org/article/S0021925820321888/pdf |
| Ending Page | 18262 |
| Page Count | 11 |
| Starting Page | 18252 |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| DOI | 10.1074/jbc.m116.737783 |
| Journal | Journal of Biological Chemistry |
| Issue Number | 35 |
| Volume Number | 291 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Chemistry Er Three-way Junction Endoplasmic-reticulum-associated Protein Degradation (erad) Protein Misfolding Ubiquitin-conjugating Enzyme (e2 Enzyme) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
