NDLI: More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species

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More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species

Content Provider	Scilit
Author	Hykollari, Alba Eckmair, Barbara Voglmeir, Josef Jin, Chunsheng Yan, Shi Vanbeselaere, Jorick Razzazi-Fazeli, Ebrahim Wilson, Iain B. H. Paschinger, Katharina
Copyright Year	2016
Description	Journal: Molecular & Cellular Proteomics N-glycosylation is an essential set of post-translational modifications of proteins; in the case of filamentous fungi, N-glycans are present on a range of secreted and cell wall proteins. In this study, we have compared the glycans released by peptide/N-glycosidase F from proteolysed cell pellets of three Penicillium species (P. dierckxii, P. nordicum and P. verrucosum that all belong to the Eurotiomycetes). Although the major structures are all within the range $Hex_{5–11}HexNAc_{2}$ as shown by mass spectrometry, variations in reversed-phase chromatograms and MS/MS fragmentation patterns are indicative of differences in the actual structure. Hydrofluoric acid and mannosidase treatments revealed that the oligomannosidic glycans were not only in part modified with phosphoethanolamine residues and outer chain och1-dependent mannosylation, but that bisecting galactofuranose was present in a species-dependent manner. These data are the first to specifically show the modification of N-glycans in fungi with zwitterionic moieties. Furthermore, our results indicate that mere mass spectrometric screening is insufficient to reveal the subtly complex nature of N-glycosylation even within a single fungal genus.
Related Links	https://www.mcponline.org/content/mcprot/15/1/73.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700520/pdf http://www.mcponline.org/article/S1535947620337099/pdf
Ending Page	92
Page Count	20
Starting Page	73
ISSN	15359476
DOI	10.1074/mcp.m115.055061
Journal	Molecular & Cellular Proteomics
Issue Number	1
Volume Number	15
Language	English
Publisher	Elsevier BV
Publisher Date	2016-01-01
Access Restriction	Open
Subject Keyword	Journal: Molecular & Cellular Proteomics Biochemical Research
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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