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Bacillus subtilis δ Factor Functions as a Transcriptional Regulator by Facilitating the Open Complex Formation
| Content Provider | Scilit |
|---|---|
| Author | Prajapati, Ranjit Kumar Sengupta, Shreya Rudra, Paulami Mukhopadhyay, Jayanta |
| Copyright Year | 2016 |
| Description | Journal: Journal of Biological Chemistry Most bacterial RNA polymerases (RNAP) contain five conserved subunits, viz. 2α, β, β′, and ω. However, in many Gram-positive bacteria, especially in fermicutes, RNAP is associated with an additional factor, called δ. For over three decades since its identification, it had been thought that δ functioned as a subunit of RNAP to enhance the level of transcripts by recycling RNAP. In support of the previous observations, we also find that δ is involved in recycling of RNAP by releasing the RNA from the ternary complex. We further show that δ binds to RNA and is able to recycle RNAP when the length of the nascent RNA reaches a critical length. However, in this work we decipher a new function of δ. Performing biochemical and mutational analysis, we show that Bacillus subtilis δ binds to DNA immediately upstream of the promoter element at A-rich sequences on the abrB and rrnB1 promoters and facilitates open complex formation. As a result, δ facilitates RNAP to initiate transcription in the second scale, compared with minute scale in the absence of δ. Using transcription assay, we show that δ-mediated recycling of RNAP cannot be the sole reason for the enhancement of transcript yield. Our observation that δ does not bind to RNAP holo enzyme but is required to bind to DNA upstream of the −35 promoter element for transcription activation suggests that δ functions as a transcriptional regulator. |
| Related Links | http://www.jbc.org/content/291/3/1064.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714191/pdf http://www.jbc.org/article/S0021925820361391/pdf |
| Ending Page | 1075 |
| Page Count | 12 |
| Starting Page | 1064 |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| DOI | 10.1074/jbc.m115.686170 |
| Journal | Journal of Biological Chemistry |
| Issue Number | 3 |
| Volume Number | 291 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Chemistry Biochemistry and Molecular Biology Bacillus Subtilis Dna Binding Protein Gram-positive Bacteria Transcription Regulation Transcriptional Regulator |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
