Loading...
Please wait, while we are loading the content...
Similar Documents
Direct lactic acid production from beech wood by transgenic white-rot fungus Phanerochaete sordida YK-624
| Content Provider | Scilit |
|---|---|
| Author | Mori, Toshio Kako, Hiroko Sumiya, Tomoki Kawagishi, Hirokazu Hirai, Hirofumi |
| Copyright Year | 2016 |
| Description | Journal: Journal of biotechnology A lactic acid (LA)-producing strain of the hyper-lignin-degrading fungus Phanerochaete sordida YK-624 with the lactate dehydrogenase-encoding gene from Bifidobacterium longum (Blldh) was constructed. When the endogenous pyruvate decarboxylase gene-knocked down and Blldh-expressing transformant was cultured with beech wood meal, the transformant was able to successively delignify and ferment the substrate. Supplementation of calcium carbonate into the culture medium, significantly increased the level of LA accumulation. Direct LA production (at 0.29g/l) from wood was confirmed, and additional inclusion of exogenous cellulase in this fermentation yielded significant further improvement in LA accumulation (up to 1.44g/l). This study provides the first report of direct production of LA by fermentation from woody biomass by a single microorganism, and indicates that transgenic white-rot fungi have a potential use for development of simple/easy applications for wood biorefinery. |
| Related Links | https://shizuoka.repo.nii.ac.jp/?action=repository_action_common_download&item_id=8039&item_no=1&attribute_id=31&file_no=1 |
| Ending Page | 89 |
| Page Count | 7 |
| Starting Page | 83 |
| ISSN | 01681656 |
| e-ISSN | 18734863 |
| DOI | 10.1016/j.jbiotec.2016.10.014 |
| Journal | Journal of biotechnology |
| Volume Number | 239 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of biotechnology Biotechnology and Microbiology Lactic Acid White-rot Fungi Woody Biomass |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
