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Real-time in vivo mitochondrial redox assessment confirms enhanced mitochondrial reactive oxygen species in diabetic nephropathy
| Content Provider | Scilit |
|---|---|
| Author | Galvan, Daniel L. Badal, Shawn S. Long, Jianyin Chang, Benny H. Schumacker, Paul T. Overbeek, Paul A. Danesh, Farhad R. |
| Copyright Year | 2017 |
| Description | Journal: Kidney International While increased mitochondrial reactive oxygen species have been commonly implicated in a variety of disease states, their in vivo role in the pathogenesis of diabetic nephropathy remains controversial. Using a two-photon imaging approach with a genetically encoded redox biosensor, we monitored mitochondrial redox state in the kidneys of experimental models of diabetes in real-time in vivo. Diabetic (db/db) mice that express a redox-sensitive Green Fluorescent Protein biosensor (roGFP) specifically in the mitochondrial matrix (db/dbmt-roGFP) were generated, allowing dynamic monitoring of redox changes in the kidneys. These db/dbmt-roGFP mice exhibited a marked increase in mitochondrial reactive oxygen species in the kidneys. Yeast NADH-dehydrogenase, a mammalian Complex I homolog, was ectopically expressed in cultured podocytes, and this forced expression in roGFP-expressing podocytes prevented high glucose–induced increases in mitochondrial reactive oxygen species. Thus, in vivo monitoring of mitochondrial roGFP in diabetic mice confirms increased production of mitochondrial reactive oxygen species in the kidneys. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656393/pdf http://www.kidney-international.org/article/S0085253817303551/pdf |
| Ending Page | 1287 |
| Page Count | 6 |
| Starting Page | 1282 |
| ISSN | 00852538 |
| e-ISSN | 15231755 |
| DOI | 10.1016/j.kint.2017.05.015 |
| Journal | Kidney International |
| Issue Number | 5 |
| Volume Number | 92 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-07-26 |
| Access Restriction | Open |
| Subject Keyword | Journal: Kidney International Urology and Nephrology Diabetic Nephropathy Reactive Oxygen Species |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nephrology |
