A novel near-infrared fluorescent probe for sensitive detection of β-galactosidase in living cells

Content Provider	Scilit
Author	Zhang, Jingtuo Li, Cong Dutta, Colin A. Fang, Mingxi Zhang, Shuwei Tiwari, Ashutosh Werner, Thomas Luo, Fen-Tair Liu, Haiying
Copyright Year	2017
Description	Journal: Analytica Chimica Acta A novel near-infrared fluorescent probe for β-galactosidase has been developed based on a hemicyanine skeleton, which is conjugated with a d-galactose residue via a glycosidic bond. The probe serves as a substrate of β-galactosidase and displays rapid and sensitive turn-on fluorescent responses to β-galactosidase in aqueous solution. A 12.8-fold enhancement of fluorescence intensity at 703 nm was observed after incubation of 10 nM of β-galactosidase with 5 μM probe for 10 min. The probe can sensitively detect as little as 0.1 nM of β-galactosidase and shows linear responses to the enzyme concentration below 1.4 nM. The kinetic study showed that the probe has high binding affinity to β-galactosidase with Km = 3.6 μM. The probe was used to detect β-galactosidase in living cells by employing the premature cell senescence model. The probe exhibited strong fluorescent signals in senescent cells but not in normal cells, which demonstrates that the probe is able to detect the endogenous senescence-associated β-galactosidase in living cells.
Related Links	https://manuscript.elsevier.com/S000326701730257X/pdf/S000326701730257X.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5455349/pdf
ISSN	00032670
e-ISSN	18734324
DOI	10.1016/j.aca.2017.02.039
Journal	Analytica Chimica Acta
Volume Number	968
Language	English
Publisher	Elsevier BV
Publisher Date	2017-05-01
Access Restriction	Open
Subject Keyword	Journal: Analytica Chimica Acta Analytical Chemistry Fluorescence Imaging Fluorescent Probe Near-infrared Emission
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry