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Differential AMP-activated Protein Kinase (AMPK) Recognition Mechanism of Ca2+/Calmodulin-dependent Protein Kinase Kinase Isoforms
| Content Provider | Scilit |
|---|---|
| Author | Fujiwara, Yuya Kawaguchi, Yoshinori Fujimoto, Tomohito Kanayama, Naoki Magari, Masaki Tokumitsu, Hiroshi |
| Copyright Year | 2016 |
| Description | Journal: Journal of Biological Chemistry $Ca^{2+}$/calmodulin-dependent protein kinase kinase β (CaMKKβ) is a known activating kinase for AMP-activated protein kinase (AMPK). In vitro, CaMKKβ phosphorylates $Thr^{172}$ in the AMPKα subunit more efficiently than CaMKKα, with a lower $K_{m}$ (∼2 μm) for AMPK, whereas the CaMKIα phosphorylation efficiencies by both CaMKKs are indistinguishable. Here we found that subdomain VIII of CaMKK is involved in the discrimination of AMPK as a native substrate by measuring the activities of various CaMKKα/CaMKKβ chimera mutants. Site-directed mutagenesis analysis revealed that $Leu^{358}$ in $CaMKKβ/Ile^{322}$ in CaMKKα confer, at least in part, a distinct recognition of AMPK but not of CaMKIα. |
| Related Links | http://www.jbc.org/content/291/26/13802.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919462/pdf http://www.jbc.org/article/S0021925820391821/pdf |
| Ending Page | 13808 |
| Page Count | 7 |
| Starting Page | 13802 |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| DOI | 10.1074/jbc.m116.727867 |
| Journal | Journal of Biological Chemistry |
| Issue Number | 26 |
| Volume Number | 291 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2016-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Chemistry Amp-activated Kinase (ampk) Ca2+/calmodulin-dependent Protein Kinase (camk) Substrate Recognition Substrate Specificity |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
