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6 HCV genotyping 9G test and its comparison with VERSANT HCV genotype 2.0 assay (LiPA) for the hepatitis C virus genotyping
| Content Provider | Scilit |
|---|---|
| Author | Chantratita, Wasun Song, Keum-Soo Ho, Choi Gun Pongthanapisith, Viroj Thongbaiphet, Nipa Wongtabtim, Garanyuta Pasomsub, Ekawat Angkanavin, Kanokwan Nimse, Satish Balasaheb Sonawane, Mukesh Digambar Warkad, Shrikant Dasharath Kim, Taisun |
| Copyright Year | 2017 |
| Description | Journal: Journal of Virological Methods In this article, we describe the 6 HCV Genotyping 9G test and its evaluation by using clinical samples and plasmid DNA standards. In tests with 981 plasmid DNA standards, the 6 HCV Genotyping 9G test showed higher than 92.5% sensitivity and 99.4% specificity. The 6 HCV Genotyping 9G test was compared with the VERSANT HCV Genotype 2.0 assay (LiPA 2.0) for detection and discrimination of HCV genotypes in clinical samples. The results of both tests were verified by genomic sequencing. The 6 HCV Genotyping 9G test demonstrated a 100% agreement with the sequencing results, which was higher than LiPA 2.0. These results indicate that the 6 HCV Genotyping 9G test can be a reliable, sensitive, and accurate diagnostic tool for the correct identification of HCV genotypes in clinical specimens. 6 HCV Genotyping 9G test can genotype six HCV types in 1 PCR in 30min after PCR amplification. The 6 HCV Genotyping 9G test, thus provide critical information to physicians and assist them to apply accurate drug regimen for the effective hepatitis C treatment. |
| Related Links | https://core.ac.uk/download/pdf/81149515.pdf |
| Ending Page | 8 |
| Page Count | 8 |
| Starting Page | 1 |
| ISSN | 01660934 |
| e-ISSN | 18790984 |
| DOI | 10.1016/j.jviromet.2016.10.009 |
| Journal | Journal of Virological Methods |
| Volume Number | 239 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2017-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Virological Methods 9g Dna Technology Hcv Genotyping Hepatocellular Carcinoma Lipa 2.0 Liver Cirrhosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology |
